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Oligonucleotide Melting Temperature
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SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (%
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SOLVED: A rudimentary method to calculate the melting temperature of DNA is an equation known as "The Wallace Rule." This equation is: Tm = 2(A + T) + 4(G + C) Where
Le calcul avancé ; . TM = tangente = yjy, MNOT = ^- à l'origine de tangente = = x le calcul de ces résultats est suffisamment evi-dent de la figure. On peut noter que thesubtangent, normale, etc., sont des valuesfor un point donné de la courbe, mais peut être ...
SOLVED: Use the Wallace Rule equation to calculate the Tm of each of the primers you designed. Tm = 2(A+T) + 4(G+C) Left primer: 5' CCTGGACACTGTAC 3' Right primer: 5' AATGTATGAACCTG 3
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